Journal: iScience

Article Title: Disuse plasticity limits spinal cord injury recovery

doi: 10.1016/j.isci.2025.112180

Figure Lengend Snippet:

Article Snippet: Polyacrylamide gel electrophoresis was performed by loading 10 μg of protein per sample into an individual lane of a precast 10-20% Tris-HCl polyacrylamide gel with three independent replications according to a randomized counterbalancing fashion blindly., Protein was transferred to a nitrocellulose membrane, blocked, and probed with primary antibodies: rabbit polyclonal anti-GluA1 (1:200; Millipore, Billerica, MA; Cat# AB1504, RRID: AB_2113602 ), rabbit polyclonal anti-GluA2 (1:200; Millipore; AB1768-25UG, RRID: AB_2247874 ), rabbit monoclonal anti-pS831-GluA1 (1:200; Millipore; Cat# 04-823, RRID: AB_1977218 ), rabbit polyclonal anti-pS880-GluA2 (1:200; Millipore; Cat# 07-294, RRID: AB_568822 ), and mouse monoclonal anti-β-Actin as a loading control (1:1500; BD Biosciences; Cat# 612657, RRID: AB_399901 ) in blocking buffer.

Techniques: Recombinant, Protease Inhibitor, Blocking Assay, Plasmid Preparation, Enzyme-linked Immunosorbent Assay, Bicinchoninic Acid Protein Assay, Software, Imaging, Inverted Microscopy, Microscopy

Journal: iScience

Article Title: Disuse plasticity limits spinal cord injury recovery

doi: 10.1016/j.isci.2025.112180

Figure Lengend Snippet:

Article Snippet: Rabbit Monoclonal Anti-pS831-GluA1 , Millipore , Cat# 04-823; RRID: AB_1977218.

Techniques: Recombinant, Protease Inhibitor, Blocking Assay, Plasmid Preparation, Enzyme-linked Immunosorbent Assay, Bicinchoninic Acid Protein Assay, Software, Imaging, Inverted Microscopy, Microscopy

Journal: Brain sciences

Article Title: Arc-Mediated Synaptic Plasticity Regulates Cognitive Function in a Migraine Mouse Model.

doi: 10.3390/brainsci13020331

Figure Lengend Snippet: Figure 4. Determination of hippocampal protein in mice. **: p < 0.01, *: p < 0.05. (A): WB results of hippocampal protein. (B): NR2B/GAPDH ratio. (C): Arc/GAPDH ratio. (D): GluR1/GAPDH ratio. (E): SYP/β-Actin ratio.

Article Snippet: Then, the sections were closed and incubated overnight with the following primary antibody: mice anti-mice Arc (1:100; Santa Cruz, USA), mice anti-mice GluR1 (1:100; Santa Cruz, USA), and rabbit anti-mice SYP (1:100, Wanleibio, China).

Techniques:

Journal: Brain sciences

Article Title: Arc-Mediated Synaptic Plasticity Regulates Cognitive Function in a Migraine Mouse Model.

doi: 10.3390/brainsci13020331

Figure Lengend Snippet: Figure 5. Determination of protein in the prefrontal cortex and hippocampus. ***: p < 0.001, **: p < 0.01, *: p < 0.05. (A): WB results of prefrontal cortex protein. (B): NR2B/GAPDH ratio. (C): GluR1/GAPDH ratio. (D): Arc/GAPDH ratio. (E): WB results of hippocampus protein. (F): NR2B/GAPDH ratio. (G): GluR1/GAPDH ratio. (H): Arc/GAPDH ratio.

Article Snippet: Then, the sections were closed and incubated overnight with the following primary antibody: mice anti-mice Arc (1:100; Santa Cruz, USA), mice anti-mice GluR1 (1:100; Santa Cruz, USA), and rabbit anti-mice SYP (1:100, Wanleibio, China).

Techniques:

Journal: Brain sciences

Article Title: Arc-Mediated Synaptic Plasticity Regulates Cognitive Function in a Migraine Mouse Model.

doi: 10.3390/brainsci13020331

Figure Lengend Snippet: Figure 9. Immunofluorescence detection of GluR1 protein in the prefrontal cortex and hippocampal CA1 region. ***: p < 0.001. The blue light point in the figure is the nucleus. The red light dot is GluR1, and the bottom left corner is a zoomed-in view of it. (A): GluR1 protein in the prefrontal cortex of the IS+S. (B): GluR1 protein in the prefrontal cortex of the IS. (C): GluR1 protein in the prefrontal cortex of the IS+M. (D): GluR1 protein in the prefrontal cortex of the Control. (E): Comparison of GluR1’s mean optical density in each group’s prefrontal cortex. (F): GluR1 expression in the hippocampal CA1 region of the IS+S. (G): GluR1 expression in the hippocampal CA1 region of the IS. (H): GluR1 expression in the hippocampal CA1 region of the IS+M. (I): GluR1 expression in the hippocampal CA1 region of the Control. (J): Comparison of GluR1’s mean optical density in each group’s hippocampal CA1 region.

Article Snippet: Then, the sections were closed and incubated overnight with the following primary antibody: mice anti-mice Arc (1:100; Santa Cruz, USA), mice anti-mice GluR1 (1:100; Santa Cruz, USA), and rabbit anti-mice SYP (1:100, Wanleibio, China).

Techniques: Control, Comparison, Expressing

Journal: Brain sciences

Article Title: Arc-Mediated Synaptic Plasticity Regulates Cognitive Function in a Migraine Mouse Model.

doi: 10.3390/brainsci13020331

Figure Lengend Snippet: Figure 10. Cellular protein expression. NGF: nerve growth factor group, n = 4; Control: Control group, n = 4; N + M: NGF + memantine group, n = 4. ***: p < 0.001, **: p < 0.01, *: p < 0.05. (A): Western blot results of cultured cells. (B): The ratio of NR2B, NR1, Arc, GluR1, SYP to β-Actin, respectively.

Article Snippet: Then, the sections were closed and incubated overnight with the following primary antibody: mice anti-mice Arc (1:100; Santa Cruz, USA), mice anti-mice GluR1 (1:100; Santa Cruz, USA), and rabbit anti-mice SYP (1:100, Wanleibio, China).

Techniques: Expressing, Control, Western Blot, Cell Culture

Journal: Frontiers in Synaptic Neuroscience

Article Title: Correlative Assembly of Subsynaptic Nanoscale Organizations During Development

doi: 10.3389/fnsyn.2022.748184

Figure Lengend Snippet: Synapse volumes increase correlatively during synaptic maturation. (A–D) Representative distribution of RIM1/2 and GluA1 under stochastic optical reconstruction microscopy (STORM). Scale 2 μm in top panels and 500 nm in lower panels. (E–G) Volumes of identified synaptic RIM1/2, GluA1, and PSD-95 clusters across different developmental stages. Numbers in bars denote the synapse numbers. (H,I) Correlations between the volumes of GluA1 and RIM1/2 clusters (H) and the volumes of PSD-95 and RIM1/2 (I) within the same synapses. Linear regressions were conducted on synapses of DIV7 (gray circles and line) and DIV18 (dark blue circles and line). Data from synapses of DIV10 and 14 were plotted with dark yellow and dark red crosses. Also refer to , and for more details on correlations and statistics. All experiments were repeated ≥3 times.

Article Snippet: For co-staining of GluA1 and RIM1/2, as both antibodies were from rabbits, staining was performed separately and the first primary antibody (GluA1) was blocked and converted with a goat anti-rabbit Fab fragment (JacksonImmuno 111-007-003) for 2 h at RT, and then recognized by secondary antibodies.

Techniques: Microscopy

Journal: Frontiers in Synaptic Neuroscience

Article Title: Correlative Assembly of Subsynaptic Nanoscale Organizations During Development

doi: 10.3389/fnsyn.2022.748184

Figure Lengend Snippet: The heterogeneity of synaptic protein distribution increases with development. (A–D) Representative density maps of synaptic GluA1 at different developmental stages. Scale bars, 200 nm. (E–G) Normalized autocorrelation functions of RIM1/2 (E) , GluA1 (F) , and PSD-95 (G) . g a above 1 suggests a significant non-uniform distribution. (H–J) Developmental changes of nanocluster number (left), normalized density within nanocluster (middle), and nanocluster volume of RIM1/2 (H) , GluA1 (I) , and PSD-95 (J) . Numbers in bars denote the synapse numbers. Also refer to for more statistical details. All experiments were repeated ≥3 times.

Article Snippet: For co-staining of GluA1 and RIM1/2, as both antibodies were from rabbits, staining was performed separately and the first primary antibody (GluA1) was blocked and converted with a goat anti-rabbit Fab fragment (JacksonImmuno 111-007-003) for 2 h at RT, and then recognized by secondary antibodies.

Techniques:

Journal: Frontiers in Synaptic Neuroscience

Article Title: Correlative Assembly of Subsynaptic Nanoscale Organizations During Development

doi: 10.3389/fnsyn.2022.748184

Figure Lengend Snippet: Correlation between presynaptic and postsynaptic protein heterogeneity in mature synapses. (A,B) Scatter plots for heterogeneity of GluA1 (A) and PSD-95 (B) against that of RIM1/2. All data points across all developmental stages could be fitted with linear functions as shown with lines. (C,D) Linear regressions of the relationships between heterogeneity of GluA1/PSD-95 and RIM1/2 at DIV7 (gray) and DIV18 (dark blue). (E) Relationship between heterogeneity and cluster volume of RIM1/2 at DIV7 (gray) and DIV18 (dark blue). Data points with cluster volume >1 × 10 7 nm 3 were fitted with linear functions. It is noted that g a of immature synapses was significantly lower than that of matured synapses. (F) Averaged g a of synapses with cluster volume of 1–4 × 10 7 nm 3 for immature (DIV7-10) and mature synapses (DIV18). Also refer to for more details. *** p < 0.001, t-test. All experiments were repeated ≥3 times.

Article Snippet: For co-staining of GluA1 and RIM1/2, as both antibodies were from rabbits, staining was performed separately and the first primary antibody (GluA1) was blocked and converted with a goat anti-rabbit Fab fragment (JacksonImmuno 111-007-003) for 2 h at RT, and then recognized by secondary antibodies.

Techniques:

Journal: Frontiers in Synaptic Neuroscience

Article Title: Correlative Assembly of Subsynaptic Nanoscale Organizations During Development

doi: 10.3389/fnsyn.2022.748184

Figure Lengend Snippet: Evolvement of trans-synaptic nano-alignment during synaptic maturation. (A–D) Representative examples of synapses with RIM1/2 (red) and GluA1 (blue) co-labeled and imaged with STORM. Thick color denotes detected nanoclusters. Scale bar, 200 nm. (E) Normalized local density of GluA1 along with distances from RIM1/2 nanoclusters for synapses at different developmental stages. (F) , Averaged enrichment of GluA1 within 50 nm from peaks of RIM1/2 nanoclusters ( n = 85, 60, 30, 54, and 54 nanoclusters). The open bar represents the enrichment indices of synapses with the position of nanoclusters randomized within synaptic clusters. (G) Fraction of nanoclusters that were enriched with protein across the cleft. (H–J) Enrichment between RIM1/2 and PSD-95 for synapses at different developmental stages. * p < 0.05, ** p < 0.01, *** p < 0.001, one-way ANOVA with Tukey's multiple comparisons test in (F,I) , z -test in (G,J) . Also refer to for more statistical details. All experiments were repeated ≥3 times.

Article Snippet: For co-staining of GluA1 and RIM1/2, as both antibodies were from rabbits, staining was performed separately and the first primary antibody (GluA1) was blocked and converted with a goat anti-rabbit Fab fragment (JacksonImmuno 111-007-003) for 2 h at RT, and then recognized by secondary antibodies.

Techniques: Labeling